Volume 12, Issue 4 (Occupational Medicine Quarterly Journal 2021)                   tkj 2021, 12(4): 1-12 | Back to browse issues page


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karimi T, Najmoddin N, Menhaje bana R. Evaluation of silica nanoparticles cytotoxicity (20-40 nm) on cancerous epithelial cell (A549) and fibroblasts cells of human normal lung fibroblast (MRC5). tkj. 2021; 12 (4) :1-12
URL: http://tkj.ssu.ac.ir/article-1-1019-en.html
Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran , najmoddin63@yahoo.com
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Evaluation of silica nanoparticles cytotoxicity (20-40 nm) on cancerous epithelial cell (A549) and fibroblasts cells of human normal lung fibroblast (MRC5)
 
Karimi T1, Najmoddin N1*, Menhaje-Bena R2
 
1 Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
 
Abstract
Introduction: Silica nanoparticles have received more attraction in medical and industrial applications due to their unique properties such as small size, the possibility of surface functionalization, ease of production, and low cost. So, it is necessary to study the respiratory toxicity of occupational exposure due to the production and increasing use of silica nanoparticles, especially in the size of 20-40 nm. 
Materials and Methods: In vitro, cytotoxicity of silica nanoparticles (SiO2 NPs) in 0.6 -3 mg/ml concentrations were evaluated on human lung epithelial (A549) and human fetal lung fibroblast (MRC5) cells by MTT assay and scanning electron microscopy (SEM) images AFTER 72 hours.
Results: The MTT assay showed that the cell viability was reduced by increasing the concentration of SiO2 NPs from 0.6 to 3 mg/ml in both types of cells. In 1.6 mg/ml concentration, the half-maximal inhibitory concentration (IC50) of SiO2 NPs on A549 cancer cells, the chance of survival is as such: %46 for A549 cancer and %70 for MRC5 normal cells. The SEM images of normal lung cells (MRC5), which have been exposed to Sio2-NP, showed that the morphology of MRCS cells has transformed from a long shape to a circular one.
Conclusion: The cytotoxicity of SiO2 NP depends on the concentration of SiO2 NP and the type of cells. By increasing SiO2 NP concentration, the cell viability decreases for both types of cells. Moreover, the cytotoxicity of SiO2 NP against cancer cells (A549) is higher than normal lung cells (MRC5).
Keywords: SiO2 NP, A549 Cells, MRC5 Cells
 
 
 
 
 
 
 
 
 
This paper should be cited as:
Karimi T, Najmoddin N, Menhaje-Bena R. Cytotoxicity of silica nanoparticles (20-40 nm) in fibroblasts cells of human normal lung (MRC5) versus cancerous one (A549). Occupational Medicine Quarterly Journal. 2021:12(4): 1-12.
 
Type of Study: Applicable | Subject: Safety and occupational accidents
Received: 2019/06/1 | Accepted: 2021/03/14 | Published: 2021/03/14

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